https://kiefferlab.com/home daily 1.0 2021-12-06 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/60b52ad4652a5e7ed4825ec2/1622485724582/alumni.png The KIEFFER Lab total number of kieffer lab trainees https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/61ae94d27e660072930cc4e7/1638831318799/publications.png The KIEFFER Lab number of publications from the lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/61ae94e4d9c3ff462450a64e/1638831333924/citations.png The KIEFFER Lab number of times lab work has been cited (google scholar) https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57c5bf56be6594b928b93af4/1472577373911/banner+cropped.jpg The KIEFFER Lab https://kiefferlab.com/publications daily 0.75 2021-12-06 https://kiefferlab.com/timothy-kieffer daily 0.75 2016-10-30 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57bb4842ff7c507a72e2973e/1477845282060/TimKieffer.jpeg Timothy Kieffer Tim kieffer https://kiefferlab.com/join-us daily 0.75 2019-03-13 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/5c898c5a0d9297d0644d7e66/1552518239798/email.png Join Us https://kiefferlab.com/funding daily 0.75 2020-07-26 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/5f1cdc86d311d6348a05b189/1595727017803/GBC_Logo_Stacked.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/5bc6165be79c704495ca7f69/1539708657977/NIH_Master_Logo_Vertical_2Color.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57e17907ebbd1a21a2961ad2/1474394399953/JDRF-2-Color-Logo.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57e175c737c58156a9892adb/1595727360864/CFI_CMYK.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57e175a937c58156a9892936/1474393574133/SCN_TransparentBkg_Bilingual.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57dc56f1e4fcb5f64a4e4f52/1474057995612/nserc_crsng_high.jpg Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57dc4193cd0f68d6291da406/1474052644119/cihr+logo.png Funding https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57bb4a0db8a79b6a706fb300/57dc20c83e00be77ca953ff3/1474052759153/MSFHR.png Funding https://kiefferlab.com/find-us daily 0.75 2019-06-03 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/5cf5b0bbdb4bbf00013aac81/1636391865688/email.png Find Us https://kiefferlab.com/what-we-do daily 0.75 2019-05-08 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57fd16292994caed2c2404b3/1476981233769/ What We Do beta-cell development in the zebrafish. one day old transgenic zebrafish embryo with developing vasculature and beta-cells labelled with green and red fluorescent protein, respectively. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/5cd317c5104c7b9c13e6d050/1557338064285/Islet_Transplantation.png What We Do Continuous blood glucose monitoring from a patient with type 1 diabetes before (left) and 9 months after islet transplantation (right), demonstrating the efficacy of a cell therapy approach. EACH LINE REPRESENTS A DIFFERENT DAY. Healthy blood glucose levels are WITHIN THE GRAY SHADED AREA. Reproduced from: LATRES E, FINAN DA, GREENSTEIN JL, KOWALSKI A, KIEFFER TJ (2019) CELL METABOLISM 29:545-563. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57e53d47ff7c50e6a8a3ed38/1474641418185/ What We Do On the cover: Kwon et al. use infrared thermal imaging to measure body surface temperature near interscapular BAT during a glucose meal in vehicle and FGF21-treated mice. Images depict body surface heat distribution of mice during such meal. The study reveals that there is a decrease in BAT temperature during glucose excursions and that FGF21 improves glucose clearance while preventing the fall in BAT temperature. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/5838bdb5893fc0a390b4ac53/1480113606497/ What We Do Stem cell differentiation for cell therapy and disease modelling. Induced pluripotent stem (iPS) cells can be derived from somatic cells, such as skin cells, of healthy individuals or patients with a disease that would benefit from cell therapy, such as type 1 diabetes. Following induction of pluripotency, stem cell differentiation protocols can program iPS cells into the desired target cell. Following stem cell differentiation, a patient in need of cell therapy may receive a cell transplant originating either from their own cells or from a healthy individual. Alternatively, where a patient’s own cells contain a disease-inducing gene mutation, gene editing with the CRISPR/Cas9 system can correct the mutation, generating normal cells that could then be transplanted back into the same patient. Gene editing of normal cells can also be employed to probe the function of individual genes in cell function and disease etiology. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57c0bf996b8f5bf91e03dd83/1472598875022/ What We Do source: International Diabetes Federation, 2015. http://www.diabetesatlas.org https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/59286fcd3a04119bb4b675dd/1495822595773/ What We Do https://kiefferlab.com/lab-members daily 0.75 2021-10-05 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57c0d5e51b631b53bedea2c1/1472597572445/GrouseMountain2015 Lab Members Grouse mountain 2015 https://kiefferlab.com/allard-cell-therapy daily 0.75 2019-05-08 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/57c6359b9f7456b07a6fb9a0/1475513098790/ Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802fd24ebbd1af6b0f8009e/1476590928306/20160816_112207_55.0+mm_023.jpg Allard Cell Therapy Lab - Peter Allard (left) and Charles Allard (right) https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802fd24e58c625ab6d9ad8b/1476594529912/20161013_150521_21+mm_065_2.jpg Allard Cell Therapy Lab - Panasonic Healthcare Cell IQ CO2 Incubators https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4bb414fb5e45ce61325/1476593686600/20161013_151449_33+mm_077.jpg Allard Cell Therapy Lab - ChemoMetec NucleoCounter NC-200 An advanced cell analyzer utilizing fluorescence imaging to characterize cell properties and perform high-precision viability and cell count determination. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4b9e4fcb5b6d1d2d709/1476588734048/20161013_150352_24+mm_060.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4b73e00be5b9a9a483c/1476588731370/20161013_145953_25+mm_048.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4b6414fb5e45ce61309/1476596592278/20161013_145437_28+mm_038.jpg Allard Cell Therapy Lab - CryoMed controlled rate freezer Programmable freezing profiles for proper and consistent preservation of cells. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4b2725e2574b75e2704/1476588727374/20161013_143857_90+mm_005.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/5802f4b2e4fcb5b6d1d2d6ee/1476588728426/20161013_145221_26+mm_036.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd43cfbe6594eca3be1251/1476596687019/20161006_150509_16+mm_369_3.jpg Allard Cell Therapy Lab - Helmer iLR125 refrigerator https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3ce5b3db2b7465c69663/1476213995859/20161006_153209_90+mm_422.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3ce3c534a593a9c3ae65/1477605902453/20161006_151905_35+mm_403.jpg Allard Cell Therapy Lab - Biorep Technologies Perifusion System https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cddc534a593a9c3adab/1478652824845/20161006_151119_16+mm_380.jpg Allard Cell Therapy Lab - Helmer Ultra-Low iUF118 -80C freezer and liquid nitrogen supply tank for MVE Chart HEco Cryogenic freezer https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cdac534a593a9c3ad84/1476597207202/20161006_145428_26+mm_341.jpg Allard Cell Therapy Lab - Zeiss Primovert Inverted Cell & Tissue Culture Microscope Allows examination of unstained cells under sterile conditions as it can remain inside the biosafety cabinet and viewed wirelessly via an iPad. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cd6b3db2b7465c695a6/1476214706740/20161006_144919_22+mm_329.jpg Allard Cell Therapy Lab - Kuhner Lab-Therm LT-XC physox shaker Built-in control of ambient oxygen levels and sensors for monitoring of sample media pH and oxygen levels. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cd29de4bb44ad7cfb94/1476214055937/20161006_144328_23+mm_317.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3ccdebbd1a584a56710b/1476214494801/20161006_144243_23+mm_309.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3ccc9de4bb44ad7cfb4e/1476213971483/20161006_143756_35+mm_284.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cc5c534a593a9c3ac1c/1478556248387/20161006_143323_26+mm_271.jpg Allard Cell Therapy Lab - MVE Chart HEco Cryogenic Freezer Uses nitrogen vapour phase for storage of cells at -190°C, eliminating potential contamination issues that may arise with liquid nitrogen. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cc4ebbd1a584a56706d/1476214485100/20161006_142855_34+mm_253.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cc1ebbd1a584a567062/1476213957153/20161006_140905_28+mm_214.jpg Allard Cell Therapy Lab https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/57fd33499de4bb0f1261ba24/57fd3cc19de4bb44ad7cfa88/1476597015179/20161006_135751_24+mm_183.jpg Allard Cell Therapy Lab - Tissue Culture Room Equipped with Baker SterilGARD biological safety cabinets. https://kiefferlab.com/lab-space daily 0.75 2016-10-19 https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5916026f15d5dbff3ab53c99/1494614658068/20170504_094415_22+mm_012.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c722e3df28f4c604322b/1476839204568/20161005_153718_35+mm_139_2.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c1121b631b37ff424e7a/1476838909438/20161005_155027_35+mm_171.jpg Lab Space - Memmert climate chamber for zebrafish embryo culture https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c1052e69cff0d1df2f02/1476838875222/20161005_151220_90+mm_102.jpg Lab Space - Zebrafish https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c102e6f2e1acf20bd013/1476838859377/20161005_150201_90+mm_056.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0f21b631b37ff424d43/1476838844297/20161005_143008_16+mm_013.jpg Lab Space - Zebrafish facility https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0ebe6f2e1acf20bcf48/1476838821309/20161005_142732_16+mm_012.jpg Lab Space - Zebrafish Facility https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0e22e69cff0d1df2dad/1476838735823/20160929_122310_16+mm_030.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0dde6f2e1acf20bced0/1476838749902/20160928_163729_16+mm_020.jpg Lab Space - Life Sciences Institute https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0cc1b631b37ff424baf/1476838784174/20160922_093447_16+mm_008.jpg Lab Space - Life Sciences Institute East Atrium https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0cae6f2e1acf20bce23/1476838767029/20160912_130845_16.0+mm_064.jpg Lab Space - Life Sciences Institute West Atrium https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0c01b631b37ff424b0c/1476838945718/20160912_111136_23.0+mm_019.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0bbe6f2e1acf20bcd73/1476838931196/20160912_110419_16.0+mm_005.jpg Lab Space https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0af2e69cff0d1df2bc2/1478815879171/20160830_114802_19.0+mm_035.jpg Lab Space - Open concept lab space Kieffer lab in the foreground https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/5806c0aef5e2310c4eb3616a/5806c0aff5e2310c4eb3616b/1476838804109/20160830_113838_24.0+mm_007.jpg Lab Space - Imaging Suite Equipped with Molecular Devices ImageXpressMicro high-content imaging systems. https://static1.squarespace.com/static/57ab73b51b631bb0ce766e58/t/5806c47846c3c450db6fbd5a/1476838671565/20160830_115444_19.0+mm_067.jpg Lab Space https://kiefferlab.com/news daily 0.75 2016-11-01